E. Coli bacteria fixing on a substrate for measurements in liquid by the method of atomic-force microscopy

For biological objects fixation in a liquid proposed to use gelling agents (gelatin and agar-agar). The studies on selection of the most appropriate mass fraction of gelatin and agar-agar in an initial solution for fixation E.Coli bacteria in the liquid media and studies of the results of fixation by method of confocal laser scanning microscopy were done. The image data of E.Coli bacterium in native state, fixated on agar-agar films in a liquid were received by method of atomic-force microscopy.