Physico-chemical parameters of erythrocytes in rats with the I type diabetes before and after oral course administration of the nanostructured complex "chitosan-insulin-gold nanoparticles"

Рresents the results of the study of parameters of erythrocytes of rats with diabetes mellitus type 1 before and after substitution therapy course with the complex "chitosan-insulin-gold nanoparticles" oral administration. The objects of the study were white mongrel female rats with alloxan diabetes mellitus. During the substitution therapy course animals with diabetes were administered with nanostructured complex orally for 10 days. The severity of the pathology and the effectiveness of the therapy were tested for the level of glucose and glycated hemoglobin in blood. To assess the possibility erythrocytes structural parameters usage in diabetes mellitus diagnostics in experimental animals, the activity of free radical oxidation, lipid composition, erythrocytes acoustic parameters and indices were determined before and after substitution therapy course. In rats with alloxan diabetes mellitus type 1, elevated concentrations of glucose, glycated hemoglobin in blood, decrease in average volume of red blood cells (MCV) and in hemoglobin amount in red blood cell (MCH), decrease in phosphatidylcholine amount in membranes of red blood cells, decrease in erythrocyte elasticity, and accumulation of sphingomyelin were detected. Substitution therapy course with the complex "chitosan-insulin-gold nanoparticles" oral administration had a corrective effect on these indicators. Changes in structural and functional characteristics of red blood cells in case of diabetes reflect both the presence of the pathology and the effectiveness of the therapy and, therefore, have a certain diagnostic significance.