Физиологические и биохимические механизмы, обеспечивающие повышенную конститутивную холодоустойчивость в растениях картофеля, экспрессирующие ген SUC2 дрожжей, кодирующий апопластическую инвертазу
Автор: Дерябин А.Н., Трунова Т.И.
Журнал: Журнал стресс-физиологии и биохимии @jspb
Статья в выпуске: 2 т.12, 2016 года.
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Экспрессия гетерологичных генов в растениях является эффективным методом улучшения нашего понимания механизмов устойчивости растений. Целью этой работы было исследование участия инвертазы клеточной стенки и апопластических сахаров в конститутивную холодостойкость растений картофеля (Solanum tuberosum L., cv. Désirée), которые экспрессировали ген SUC2 дрожжей, кодирующий апопластическую инвертазу. В качестве контроля служили WT-растения картофеля. Увеличение существенной активности инвертазы клеточной стенки в листьях трансформированных растений свидетельствует о значительных изменениях в метаболизме клеточного углевода и регулятивной функции этого фермента. Активность дрожжевой инвертазы изменила состав внутриклеточных сахаров в листьях растения трансформированного картофеля. Общее содержание сахаров (сахароза, глюкоза, фруктоза) в листьях и апопласте было выше у трансформантов по сравнению с WT-растениями. Наши данные свидетельствуют о более высокой конститутивной устойчивости трансформантов к тяжелым условиям гипотермии по сравнению с WT-растениями. Этот факт позволяет нам рассматривать инвертазу клеточной стенки как фермент углеводного обмена, играющий важную регуляторную роль в метаболической сигнализации при формировании повышенной устойчивости растений к низкой температуре. Таким образом, линия картофеля с интегрированным геном SUC2 является удобным инструментом для изучения роли апопластической инвертазы и продуктов ее активности в процессе роста, развития и формирования конститутивной резистентности к гипотермии.
Короткий адрес: https://sciup.org/14323990
IDR: 14323990
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