Physiological changes of microreological erythrocyte properties in creating dislipidemia in rats at late stages of ontogenesis

The state of spontaneous aggregation and erythrocyte cytoarchitectonics at the earliest stages of dyslipi-demia development has not sufficiently been studied. Experimental studies on the early dyslipidemia influence on erythrocytes are of great practical interest. The objective is to trace the dynamics of erythrocyte microrheological properties in the model of dyslipidemia in older rats. Materials and Methods. The study was conducted on healthy Wistar rats. The experimental group enrolled 105 male rats (34 rats aged 12 months, 32 rats aged 18 months and 39 rats aged 24 months). All the rats had alimentary modelled dyslipidemia. The control group enrolled 91 male rats (30 rats aged 12 months, 32 rats aged 18 months and 29 rats aged 24 months). Biochemical, hematological and statistical methods were applied. Results. With aging the activity of free radical lipid oxidation in blood plasma increased in experimental animals due to a decrease in its antioxidant activity. Lipid peroxidation was also more obvious with aging in erythrocytes of experimental rats, while the activity of superoxide dismutase and catalase in erythrocytes decreased. The experimental rats also demonstrated a decrease in the number of discoid red blood cells and a significant increase in the number of their reversibly and irreversibly changed forms as compared with the controls. This was accompanied by a more pronounced increase in the aggregation activity of erythrocytes in experimental rats in comparison with the controls. Conclusion. Experimental modelled dyslipidemia in rats in the late ontogenesis has shown the possibility of developing more severe changes in biochemical and hematological indicators with aging. Developing disorders of erythrocyte microrheological properties in experimental rats exceeded the age-related changes in controls. The data obtained should draw the clinician's attention to a stricter control of blood lipid levels in patients of pre-retirement and retirement age, since with aging dyslipidemia becomes more dangerous due to a decrease in aggregation activity and cytoarchitectonics of red blood cells and high risk of thrombophilia.