Formation of biofilms by uropatogenic strains of Escherichia coli on variousabiotic surfaces

Studied the formation of biofilms of clinical strains of uropathogenic Escherichia coli (UPEC) on polystyrene and abiotic surfaces of medical appointments - latex, polyvinyl chloride (PVC), silicone. Biofilms were heavier on the polystyrene than on the catheters PVC (W-test: p=0.028) and silicone (p=0.027), but not latex (p>0.05). Formed on the surface of urological catheters by UPEC biofilm was heavier on the latex (latex vs PVC p=0.028; latex vs silicone p=0.019). The improved hydrophilicity of the surface attacked, analyzed on the adsorption of naphthalene reduced the effectiveness of its colonization of bacteria (latex > polystyrene > PVC > silicone). There was a strong positive relationship between the biomass of the biofilm and cell wall hydrophobicity on hydrophobic carriers (latex: rs=0.675; polystyrene: rs=0.713), was reduced in more hydrophilic surfaces (PVC: rs=0.334; silicone: rs=0.371). UPEC number of bacteria in the composition of the biofilm was different on all surfaces, the maximum number of viable cells detected on polystyrene and silicone. It is shown that the most massive biofilm was formed on polystyrene when glucose concentration is 3.5% and at pH 6.0 and 7.0.