Method ECD / ETD fragmentation of the phosphopeptides in modern tandem mass spectrometry

Автор: Krasnov I.A., Gavrik M.A., Podolskaya E.P., Krasnov N.V.

Журнал: Научное приборостроение @nauchnoe-priborostroenie

Рубрика: Масс-спектрометрия

Статья в выпуске: 1 т.23, 2013 года.

Бесплатный доступ

The paper presents features and the application of peptides fragmentation method in tandem mass spectrometry, in particular fragmentation techniques CID, ECD and ETD, and their combinations. Mechanism of fragmentation, sample preparation methods and sample introduction in relation to the method of ECD / ETD are analyzed. The possibility to use common proteomic databases for solving phosphoproteomic tasks is shown. The conclusions about the effectiveness of CID / ETD fragmentation methods combination to expand amino acid sequence restore capacity of the phosphopeptides are made.

Mass spectrometry, tandem mass spectrometry, ion source, fragmentation, phosphopeptide, proteomics

Короткий адрес: https://sciup.org/14264843

IDR: 14264843

Статья обзорная