Fuelling of TCA cycle in hepatic cells Marwari goat during ambient temperature associated stress
Автор: Kataria N., Kataria A.K., Joshi A.
Журнал: Журнал стресс-физиологии и биохимии @jspb
Статья в выпуске: 4 т.6, 2010 года.
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The present study was launched to assess the effect of extreme ambient temperature associated stress on fuelling of TCA cycle in hepatic cells of Marwari goat. Based on the fact that whenever a hepatocyte needs fuel for TCA cycle, the activity of enzyme glutamate dehydrogenase (GD) increases making alpha-ketoglutarate available for TCA cycle, 600 apparently healthy Marwari goats of either sex, between 6 months to 3 years of age were screened and blood samples were collected during moderate, cold and hot ambient temperature periods to determine the serum glutamate dehydrogenase enzyme and glucose concentration. The mean value of serum GD was significantly (p≤0.05) higher during cold and hot ambient temperature periods in comparison to overall moderate mean value. However, the rise was greater in cold (2.20 times) than hot ambient temperature (1.19 times). The serum GD activity was higher in male and younger animals. Serum glucose concentration showed a reverse trend as compared to serum GD activity. The results indicated that in cold condition associated stress the fuelling to TCA cycle was more than moderate and hot ambient temperature periods. Serum GD activity was also found related with glucose homeostasis. Further the study has shown that variations in the enzyme levels are not always pathological and while interpreting clinical data, a clinician must consider these variations.
Ambient temperature, cold, glucose, glutamate dehydrogenase, hot, marwari goat
Короткий адрес: https://sciup.org/14323501
IDR: 14323501
Список литературы Fuelling of TCA cycle in hepatic cells Marwari goat during ambient temperature associated stress
- Banerjee, S., Schmidt, T., Fang, J., Stanley, C.A., Smith, T.J.(2003). Structural Studies on ADP Activation of Mammalian Glutamate Dehydrogenase and the Evolution of Regulation. Biochem. 42, 3446-3456.
- Boyd, J. W. (1962). The comparative activity of some enzymes in sheep, cattle and ratsnormal serum and tissue levels and changes during experimental liver necrosis. Res.Vet.Sci. 3,256-268.
- El Samani, F., Mahmoud O.M., Fawi, M.T., Gameel, A.A., Haroun, E.M. (1985). Serum enzyme activity and bilirubin concentration in sheep experimentally infected with Fasciola gigantica. J. Comp. Pathol. 95(4), 499-503.
- Kataria, N., Kataria, A.K., Agarwal, V.K., Garg, S.L., Sahani, M.S. and Singh, R. (2000). Effect of water restriction on serum aldosterone and cortisol in dromedary camel during winter and summer. J. Camel Prac. Res. 7,1-7.
- King, J. (1965). In, Practical clinical enzymology. D. Van Nostrand Company Ltd., London. pp 25-301.
- Lehninger, A.L., Nelson,D.L. and Cox, M.M. (1993). In, Principles of Biochemistry. 2nd edn. Worth publishers, New York. pp 400-787.
- Locher,L., Rudovsky, A. and Furll, M. (2008). Investigations on the antioxidative metabolism during the periparturient period in dairy goats with special regard to seasonal differences in feeding and management. Beri Munch Tierarzti Wochenschr. 121 (9-10), 341-348.
- Maechler, P. and Wollheim, C.B. (1999). Mitochondrial glutamate acts as a messenger in glucose-induced insulin exocytosis. Nature. 402,685-689.
- Oser, B.L. (1976). In, Hawks physiological chemistry. 14th ed. Tata McGraw Hill Publishing Co. Ltd., New Delhi. Pp 975-1152.
- Sener, A. and Malaisse, W.J. (1980).L-leucine and a nonmetabolized analogue activate pancreatic islet glutamate dehydrogenase. Nature. 288,187-189.
- Snedecor, G. W. and Cochran, W. G. (1967).In, Statistical Methods. 6th ed. New Delhi.
- Stillman, T.J., Baker, P. J., Britton, K.L. and Rice, D.W. (1993). Conformational flexibility in glutamate dehydrogenase: role of water in substrate recognition and catalysis. J. Mol. Biol. 234(4), 1131-1139.
- Tanizawa, Y., Nakai, K., Sasaki,T., Anno,T., Ohta,Y., Inoue,H., Matsuo,K., Koga,M., Furukawa,S. and Oka,Y. (2002). Unregulated elevation of glutamate dehydrogenase activity induces glutaminestimulated insulin secretion identification and characterization of a GLUD1 gene mutation and insulin secretion studies with MIN6 cells overexpressing the mutant glutamate dehydrogenase. Diabetes. 51,712-717.
- Tennant, B.C. (1999). Hepatic function. In, Clinical Biochemistry of Domestic Animals. 5th edn. Harcourt Brace & Company, Asia Pvt. Ltd.
