Functional characteristics of peripheral blood mononuclear cells after administration of granulocyte colony-stimulating factor in patients with chronic heart failure

It is shown that the introduction of granulocyte colony stimulating factor (G-CSF) in patients with chronic heart failure increases the functional activity of peripheral blood mononuclear cells (MNC). Enriching peripheral blood MNCs with progenitor cells (PC) by mobilization of bone marrow leads to an increase of their proliferative capacity both under spontaneous culture conditions and when stimulated with ConA mitogen and cytokines G-CSF. Proliferative activity of MNCs is found to increase, as the number of CD34+ PC starts growing. It is also noted that MNCs produce a wide range of cytokines with regulatory action after mobilization in a 48-hour culture in vitro. The findings suggest the feasibility of using MNCs after G-CSF mobilization for stimulation of reparative processes in the myocardium.