Genetic diversity Corylus avellana L. in Lesser Caucasus of Azerbaijan

Автор: Ibragimov Zakir, Alizade Roza

Журнал: Бюллетень науки и практики @bulletennauki

Рубрика: Биологические науки

Статья в выпуске: 10 т.5, 2019 года.

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The article is devoted to the results of a study of the genetic polymorphism of hazel ( Corylus avellana (L.) H. Karst.), growing in the Lesser Caucasus within Azerbaijan. For the analyzes used nuclear DNA extracted from sheet material. DNA extraction, PCR and ISSR analyzes were carried out according to standard methods (CTAB, PCR, ISSR protocols). According to the results of analyzes using 4 ISSR primers, the number of identified fragments was 42, which corresponds to 9-12 loci per primer (~10.8). Of the 42 fragments identified, 34 (80.95%) are polymorphic, and 8 (19.05%) are monomorphic. The number of polymorphic loci varies in the range of 7-10. With the smallest number of amplified loci in the UBC811 primer, the largest number of them occurs in the UBC827 primer. Depending on the primer, the number of amplified polymorphic loci varies within 63-90%. The level of ISSR primer polymorphism is on average 86% (75-96%). The average value of the actual heterozygosis (H0) is 0...

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Issr-праймеры, hazel, population, genetic polymorphism, issr primers, cluster analysis

Короткий адрес: https://sciup.org/14115093

IDR: 14115093   |   DOI: 10.33619/2414-2948/47/05

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