Characterization of intercellular relationships in HIV/TB co-infection taking into account the expression of biomarkers CD3+, CD7+, CD10+ in the lungs

The study of morphological changes directly in areas of inflammatory response in HIV/TB coinfection allow to estimate the functional activity and intercellular relationships of immune cells. The purpose of the study was to assess the degree of expression of CD3+,CD7+,CD10+ biomarkers in foci of granulomatous inflammation in lung tissues of patients who died of HIV / TB co-infection. Autopsy material (n=30) of patients with HIV/TB co-infection was studied, stage 4B-5 - group 1. In the comparison group - a group 2 - includes autopsy cases (n=30) with mono-infection with TB. Immunohistochemical investigation to determine the expression of biomarkers of CD3+,CD7+,CD10+ was performed with using rabbit monoclonal antibodies (Epitomics, Abcam). The severity of biomarker expression was assessed with the use of Image Analysis with subsequent morphometric analysis. The author showed a reduction in the number of morphologically mature CD3+ T-lymphocytes and an increase in the number of immature CD7+ T-lymphocytes mainly in foci of productive inflammation, indicating a profound inhibition of the immune response in HIV infection in the terminal stages of immunodeficiency, with the development of tuberculosis...