Characteristics of tautomeric forms of potassium orotate obtained by mechanical activation

The purpose of the study: to obtain mechanoactivated tautomeric forms of potassium orotate, to evaluate the physical and chemical characteristics of tautomers and the effectiveness of their action on the body of laboratory animals. Research methods: mechanical activation of potassium orotate in a spherical planetary mill, investigation of powders by atomic force microscopy, X-ray phase analysis, NEXAFS spectrum, and investigation of the effects of the use of powders of various tautomeric forms of potassium orotate on animals. It was found that the mechanical activation of the initial oxo-form during 1 h led to the formation of the hydroxy-form, after 6h - the dihydroxy-form, which differs in physical and chemical properties. In animal experiments, a higher efficiency of mechanoactivated drugs (hydroxy-form > dihy-droxy-form) were found to be more effective than the original oxo-form. The obtained data can be used as a basis for clinical testing of the hydroxyl-form of orotic acid, as the most active form of the drug.