Heavy metals pollution and its genotoxicity assessment through the green marine Ulva lactuca (Chlorophyceae) algae in the Lattakia-coastline, Syria
Автор: Saleh Basel
Журнал: Журнал стресс-физиологии и биохимии @jspb
Статья в выпуске: 1 т.16, 2020 года.
Бесплатный доступ
The current study was conducted to investigate pollution level in Lattakia-coastline, Syria using Ulva lactuca (Chlorophyta) algae as bioindicator for sea water pollution based on Zn, Pb, Cu and Cd concentration using Atomic Absorption Spectrometry (AAS) technique. On the other hand, to investigate pollution genotoxicity on U. lactuca algae based on Genomic Template Stability (GTS%) estimations yielded by Touch-down Directed Amplification of Minisatellite DNA (Td-DAMD) marker. AAS analysis indicated that the Lattakia coastline is not polluted by the mentioned pollutants (Zn, Pb, Cu and Cd) at least regarding these metals. As for GTS%, it is not correlated with observed pollutants concentration in the mentioned sites. Further studies focused especially on determination of other elements than those studied herein and investigation their influence on DNA changes profile are needed.
Ulva lactuca algae, heavy metals pollution, atomic absorption spectrometry (aas) technique, genomic template stability (gts%)
Короткий адрес: https://sciup.org/143171151
IDR: 143171151
Список литературы Heavy metals pollution and its genotoxicity assessment through the green marine Ulva lactuca (Chlorophyceae) algae in the Lattakia-coastline, Syria
- Abacus. (1996) Concept, Statview 4.5 statistical program. Berkeley, Calif. Abacus Concepts
- Akcali I, Kucuksezgin F. (2011) A biomonitoring study: Heavy metals in macroalgae from eastern Aegean coastal areas. Mar Pollut Bull 62(3), 637-645
- Allam, H., Aouar, A., Benguedda, W. and Bettioui, R. (2016) Use of sediment and algae for biomonitoring the coast of Honaïne (Far West Algerian). Open J Ecol 6, 159-166
- Apaydın G, Aylıkcı V, Cengiz E, Saydam M, Küp N, Tıraşoğlu E. (2010) Analysis of metal contents of seaweed (Ulva lactuca) from Istanbul, Turkey by EDXRF. Turk J Fish Aquat Sci 10, 215-220
- Annabi A, Zrig A, Jeddi K, Saleh B (2018) Assessment of trace metal accumulation in green algae Ulva from the South Eastern coast of Tunisia. J Marine Biol Ocean 7(2), 1000193
- Aras S., Beyaztaş T., Cansaran-Duman D., Gökçe-Gündüzer E. (2011) Evaluation of genotoxicity of Pseudevernia furfuracea (L.) Zopf by RAPD analysis. GenMol Res 10 (4), 3760-3770
- Atienzar FA, Venir P, Jha AN, Depledge MH. (2002)Evaluation of the random amplified polymorphic DNA (RAPD) assay for the detection of DNA damage and mutations. Mut Res 521, 151-163
- Cadar E, Mustafa A, Tomescu A, Cherimi M. (2018) Studies regarding polluting agents in Black Sea algae. J Sci Art 1(42), 255-264
- Chakraborty S, Owens G. (2014) Metal distributions in seawater, sediment and marine benthic macroalgae from the South Australian coastline. Int J Environ Sci Technol 11, 1259-1270
- Chakraborty S, Bhattacharya T, Singh G, Maity JP. (2014) Benthic macroalgae as biological indicators of heavy metal pollution in the marine environments: a biomonitoring approach for pollution assessment. Ecotoxicol Environ Saf 100, 61-68
- Chen Q, Pan X-D, Huang B-F, Han J-L. (2018) Distribution of metals and metalloids in dried seaweeds and health risk to population in southeastern China. Sci Rep 8, 3578
- Dourado PLR, da Rocha1MP, Roveda LM, Junior JLR, Cândido LS, Cardoso CAL, Morales MAM, de Oliveira KMP, Grisolia AB. (2017) Genotoxic and mutagenic effects of polluted surface water in the midwestern region of Brazil using animal and plant bioassays. Gen Mol Biol 40 (1), 123-133
- Doyle JJ, Doyle JL. (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19, 11-15
- Gaudry A., Zeroual S., Gaie-Levrel F., Moskura M., Boujrhal F-Z., Cherkaoui El Moursli R., Guessous A., Mouradi A., Givernaud T., Delmas R. (2007) Heavy metals pollution of the Atlantic marine environment by the Moroccan phosphate industry, as observed through their bioaccumulation in Ulva Lactuca. Water Air Soil Pollut 178, 267-285
- Ince AG, Karaca M. (2012) Species-specific touch-down DAMD-PCR markers for Salvia species. J M Plant Res 6(9), 1590-1595
- Jamers, A., Blust, R., De-Coena, W., Griffinab, J.L. and Jonesco, O.A.H. (2013). An omics based assessment of cadmium toxicity in the green alga Chlamydomonas reinhardtii. Aquat Toxicol 126 (1), 355- 364
- Jayasinghe GDTM, Jinadasa BKKK, Chinthaka SDM. (2018) Nutritional composition and heavy metal content of five tropical seaweeds. Open Sci J Anall Chem 3(2), 17-22
- Laib, E., Leghouchi, E. (2012). Cd, Cr, Cu, Pb, and Zn concentrations in Ulva lactuca, Codium fragile, Jania rubens, and Dictyota dichotoma from Rabta Bay, Jijel (Algeria). Environl Monit Assess 184, 1711-1718
- Mohamed L.A., Khaled A. (2005) Comparative study of heavy metal distribution in some coastal seaweeds of Alexandria, Egypt Chem Ecol 21(3), 181-189
- Saleh, B. (2015) Detection of genetic variations in marine algae Ulva lactuca (Chlorophyta) induced by heavy metal pollutants. J Stress Physiol Biochem, 11 (3), 26-37
- Saleh, B. (2016a)Genetic variation in Ulva lactuca (Chlorophyceae) marine algae under cadmium stress. J Bioinnov, 5 (5), 673-685
- Saleh, B. (2016b) DNA changes in cotton (Gossypium hirsutum L.) under salt stress as revealed by RAPD marker. Adv in Horticul Sci 60(1), 13-21
- Saleh B. (2018a) Algae in pharmacology and medicine applications: A review. J Bioinnov 7 (6), 878-907
- Saleh B. (2018b) Lead genotoxicity in Ulva lactuca (Chlorophyta) seaweed as revealed by RAPD marker. J Bioinnov 7 (5), 790-803
- Topcuoğlu S, Kılıç Ö, Belivermiş M, Ergül HA and Kalaycı G. (2010) Use of marine algae as biological indicator of heavy metal pollution in Turkish marine environment. J Black Sea/Med Environ 16(1), 43-52
- van Ginneken, V. and de Vries, E. (2018) Seaweeds as biomonitoring system for heavy metal (HM) accumulation and contamination of our oceans. Am J Plant Sci, 9, 1514-1530
- Wong MH, Kwok TT, Ho KC. (1982) Heavy metals in Ulva lactuca collected within Tolo Harbour, an almost landlocked sea. Hydrobiol Bull. 16 (2-3), 223-230