Hydrogen peroxide and phenylalanine ammonialyase as signalling molecules in barley leaves challenged with Cochliobolus sativus
Автор: Al-Daoude Antonious, Jawhar Mohammed, Al-Shehadah Eyad, Shoaib Amina, Arabi Mohammed Imad Eddin
Журнал: Журнал стресс-физиологии и биохимии @jspb
Статья в выпуске: 2 т.18, 2022 года.
Бесплатный доступ
Hydrogen peroxide (H2O2) and phenylalanine ammonia-lyase ( PAL ) have been reported as important signaling molecules during plant resistance against many fungal pathogens. In this study, the relative contributions of H2O2 and PAL were investigated at early time periods of barley infection with Cochliobolus sativus , the causal agent of spot blotch disease. H2O2 activity was observed in leaf tissues 24 hours post inoculation (hpi) and was accompanied with an increase in PAL expression in resistant and susceptible genotypes. However, the resistant genotype ‘Banteng’ contained higher levels of H2O2 and PAL , as compared with the susceptible one ‘WI 2291’. Results demonstrated that the cooperative function of H2O2 and PAL in barley responses to C. sativus appeared to be dependent on the plant genotype, and it is hypothesized that the peak of activity of PAL at 48h and 72 h, and the rapid increase in H2O2 24 h in resistant and susceptible genotypes are considered general defense responses.
Hordeum vulgare, cochliobolus sativus, h2o2, pal expression, real time pcr
Короткий адрес: https://sciup.org/143178805
IDR: 143178805
Список литературы Hydrogen peroxide and phenylalanine ammonialyase as signalling molecules in barley leaves challenged with Cochliobolus sativus
- Al-Daoude A, Jawhar M, Arabi MIE. 2013. Hydrogen peroxide induction in barley-Cochliobolus sativus interaction. J. Plant Pathol., 95: 197-199.
- Arabi MIE, Jawhar M. 2003. Pathotypes of Cochliobolus sativus (spot blotch) on barley in Syria. J. Plant Pathol., 85: 193-196
- Arabi MIE, Jawhar M. 2004. Identification of Cochliobolus sativus (spot blotch) isolates expressing differential virulence on barley genotypes in Syria. J. Phytopathol., 152: 461-464.
- Al-Sadi AM. 2021. Bipolaris sorokiniana-induced black point, common root rot and spot blotch diseases of wheat: A review. Front. Cell. Infect. Microbiol., 11: 584899.
- Brennan T, Frenkel C. 1977. Involvement of hydrogen peroxide in the regulation of senescence in pear. Plant Physiology 59: 411-416.
- Chaman ME, Copaja SV, Argandona VH. 2003. Relationships between salicylic acid content, phenylalanine ammonia-lyase (PAL) activity, and resistance of barley to aphid infestation. J. Agri. Food Chem., 51: 2227-2231.
- Delledonne M, Xia YJ, Dixon RA, Lamb C. 1998. Nitric oxide functions as a signal in plant disease resistance. Nature, 394: 585-588.
- Derveaux S, Vandesompele J, Hellemans J. 2010. How to do successful gene expression analysis using real-time PCR. Methods, 50: 227-230.
- Desikan R, Reynolds A, Hancock JT, Neill SJ. 1998. Harpin and hydrogen peroxide both initiate programmed cell death but have differential effects on defence gene expression in Arabidopsis suspension cultures. Biochem. J., 330: 115-120.
- Fetch TG, Steffenson BJ. 1999. Rating scales for assessing infection responses of barley infected with Cochliobolus sativus. Plant Dis., 83: 213-217.
- Juan CA, Pérez de la Lastra, JM, Plou FJ, Pérez-Lebeña E. 2021. The chemistry of reactive oxygen species (ROS) revisited: Outlining their role in biological macromolecules (DNA, lipids and proteins) and induced pathologies. Int. J. Mol. Sci. 22: 4642.
- Gupta S, Loughman R, Platz GJ, Lance R, Jones M. 2001. Journey of net blotch from pathotype diversity to useful resistance in barley. Proceedings of the 9th Australian Barley Technical Symposium, Melbourne, Australia.
- Kim DS, Hwang BK. 2014. An important role of the pepper phenylalanine ammonia-lyase gene (PAL1) in salicylic acid-dependent signalling of the defence response to microbial pathogens. J. Exp. Bot., 65: 2295-2306.
- Kumar J, Schafer P, Huckelhoven R, Langen G, Baltruschat H, Stein E, Nagarajan S, Kogel HK. 2002. Bipolaris sorokiniana, a cereal pathogen of global concern: cytological and molecular approaches towards better control. Mol. Plant Pathol. 3: 185-195.
- Leng Y, Wang R, Ali S, Zhao M, Zhong S. 2016. Sources and genetics of spot blotch resistance to a new pathotype of Cochliobolus sativus in the USDA small grains collection. Plant Dis., 100: 1988-1993.
- Livak K J, Schmittgen TD. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2 (-Delta Delta C (T)) Method. Methods, 25: 402-408.
- Rehman S, Gyawali S, Amri A, Verma RPS. 2020. First report of spot blotch of barley caused by Cochliobolus sativus in Morocco. Plant Dis., 104: 3.
- Sahebani N, Hadavi N. 2009. Induction of H2O2 and related enzymes in tomato roots infected with root knot nematode (M. javanica) by several chemical and microbial elicitors. Biocontrol. Sci. Technol., 19: 301-313.
- Thordal-Christensen H, Zhang Z, Wei Y, Collinge D B. 1997. Subcellular localization of H2O2 in plants. H2O2 accumulation in papillae and hypersensitive response during the barley—powdery mildew interaction. Plant J., 11: 1187-94.
- Vogt T. 2010. Phenylpropanoid biosynthesis. Mol. Plant, 3: 2-20.
- Yadav V, Wang Z, Wei C, et al. 2020. Phenylpropanoid pathway engineering: An emerging approach towards plant defense. Pathogens. 9: 312.
