Identification of Bordetella bronchiseptica bacteria with the help of polymerase chain reaction in mono and multyplex format
Автор: Vasilyeva Yu.B.
Журнал: Вестник аграрной науки @vestnikogau
Статья в выпуске: 6 (45), 2013 года.
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The article gives the results of the original research aimed at working out molecular-genetic methods for the identification of Bordetella bronchiseptica. The parameters for conducting polymerase chain reaction (PCR) with the worked out systems of primers within using monoplex and multiplex format are suggested. The optimal methodology of singling out B. bronchiseptica DNA is selected; the main parameters of the reaction are validated. The research was performed with the financial help of the government represented by the Ministry of Science and Education of the Russian Federation within the implementation of the Federal Target Project “Scientific and Scientific-Pedagogical Staff of the Innovative Russia” for the years 2009-2013 (agreement № 8267 from 10.08.2012).
Bordetella bronchiseptica, bordetellosis, polymerase chain reaction
Короткий адрес: https://sciup.org/147124138
IDR: 147124138
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