Identification of FGF21 gene polymorphism in Holstein cattle population of Tatarstan

The present study is dedicated to genotyping of Holstein cattle whit using PCR-RLFP method and cattle pituitary-specific transcription factor-1gene allele and genotype diversity monitoring in the different breeds. 148 Holstein cows of Integrated Agricultural Production Centre «Stud farm named after Lenin» of Atninsky district of Republic of Tatarstan were genotyped for the study. Blood sample DNA testing showed that the FGF21 gene is polymorphic for the population under the research. In the course of work the following allelic variants and genotypes were identified: C - 0.642 and T - 0.358; CC - 28.4 % (42 animals), TC - 71.6 % (106 animals), TT - 0.0 % (0 animals). The chi-square method testing between the observed and expected genotype distribution indicates a violation of genetic equilibrium in the population under study.