Identification of PIT-1 gene polymorphism in Holstein cattle population of Tatarstan

The present study is dedicated to genotyping of Holstein cows-heifers whit using PCR-RLFP method and cattle pituitary-specific transcription factor-1gene allele and genotype diversity monitoring in the different population. 330 Holstein heifers of Integrated Agricultural Production Centre «Stud farm named after Lenin» of Atninsky district of Republic of Tatarstan were genotyped for the study. Blood sample DNA testing showed that the PIT-1 gene is polymorphic for the population under the research. In the course of work the following allelic variants and genotypes were identified: A - 0.323 and B - 0.677; AA - 11.9% (39 animals), AB - 40.5% (135 animals), BB - 47.6% (156 animals). The chi-square method testing between the observed and expected genotype distribution indicates genetic equilibrium in the population under study. For all groups of Holstein cattle the prevalence of the B allele over the A allele.