Immunological pathways of development of experimental acquired toxic hemolytic anemia

Toxic hemolytic anemia, induced by drugs and industrial reagents, is provided by combination of different pathogenetic mechanisms. 2-butoxyethanol, a widely used in chemical industry reagent and strong hemolytic poison, was taken for modeling toxic hemolytic anemia. 2-butoxyethanol was injected to white nonlinear rats intraperitoneally. We were taken venous blood for erythrocyte suspension before the experiment. After 10 days we carried out serum agglutination test with autologous erythrocytes. In peripheral blood was found significant decreasing in hemoglobin level and reducing of red blood cells number, increasing in number of reticulocytes. In serum agglutination test was observed the presence of antibodies to own erythrocytes both in group with injection of 2-butoxythanol and in group with injection of 2-butoxyethanol in combination with acute cold stress. It shows the development of autoimmune reaction to own red blood cells. In serum agglutination test with heterologous erythrocytes reaction was positive in control group with average values log - 2,5±1,3. It is explained by genetical identity in outbred population of laboratory rats.