Immunological status of repairing young cattle on the background of application of different doses of biostimulant tissue

The research objective was studying the influence of different doses of tissue biostimulant on immune status of repair young growth of cattle of Priobsky kind of black and motley breed. The experiment was made in 2020 on the basis of JSC “Uchkhoz ‘Prigorodnoye’", Industrial district of Barnaul, Altai Region where by the principle of analogs 4 experimental groups of repair heifers up to 10 heads in each were created. At the selection of animals the age (1 month) and live weight (51.0 kg) were considered. The experiment lasted for 14 days. Experimental batch of tissue biostimulant was made from offal and slaughterhouse waste of antler deer using a patented technology. Placenta, uterus with fetus, lymph nodes, spleen and liver were the ingredients of tissue biostimulant. The quality control for the toxicity and reactogenicity was carried out on white mice in accordance with State Standard 31926-2013 “Medicines for veterinary use. The methods for determining harmlessness "and methodological instructions" On bacteriological control of sterility of veterinary biological products "No. 115-6A dated 03.06.1980. Control animals were given physiological solution in the dose of 3.0 ml/head once subcutaneously, in I experimental group - tissue biostimulant in the dose of 2.0 ml/ head in II - 3.0 ml/head, in III - 4.0 ml/head. Introduction of tissue biostimulant to repair young cattle promoted the formation of T- and B-systems of the immunity. As an optimum dose of studied preparation it is necessary to consider 3 ml/head promoting the increase in relative quantity of bE-ROCK “activated lymphocytes” by 1.5 % (p ≤0.05), rE-ROCK “inducers-helpers” - by 1.8 (p ≤0.05), EM-ROCK “B-lymphocytes” - by 1.7 (p ≤0.05), absolute content of bE-ROCK - by 48.1 (p ≤0.05) and EM-ROCK - by 46.4 % (p ≤0.05).