The induction of antioxidative enzymes in plants (Pisum sativum) by excess ammonium-chloride

Investigated action of ammonium-chloride (10-6-10-2 М) on peas at addition on medium of cultivation. The augmentation of concentration of this salt result ined to retardation of growth plants, decrease of the contents of protein and of chlorophyll in leaves and depression of rate of synthesis ATP in the isolated chloroplasts. At excess of ammonium (2,5 x 10-2 M) and long action of smaller concentrations (10-6 -10-4 М) the activity of enzymes of a superoxide dismutase, catalase and peroxidase in leaves and roots was enlarged. The comparison soluble (cytosoling) and hardsoluble (membrane-bound) proteinaceous fractions, and also subcellular of fragments, secured of young growth peas, has shown activating enzymes by ammonium-chloride mainly in membranous structures (nuclei, subparticles, mitochondrions and chloroplasts). The activation antioxidative of enzymes in plants of peas is discussed in connection with by membranotropism action of ammonium-chloride. The conclusion about intensifying of oxidative activity of peroxidase is made at toxicity excess salt.