Induction of apoptosis in B16 melanoma cells under the action of drug containing tumor necrosis factor alpha as a part of virus-like particles in vitro

Aim: to evaluate the antitumor activity of the drug containing TNF-alpha and high-polymer double-stranded RNA (dsRNA) in the composition of virus-like particles (VLP-TNF-alpha) on B16-F10 melanoma cells. Material and Methods. Analysis of the anti-proliferative effect of VLP-TNF-alpha as well as its components, TNF-alpha and dsRNA, was carried out using the MTT-test. Apoptosis of melanoma cells was assessed by flow cytofluorimetry with FITC-annexin V. Results. It was shown that the cytotoxic effect of the drug containing the combination of TNF-alpha and dsRNA on melanoma cells significantly exceeded the total cytotoxic effect of TNF-alpha or dsRNA alone (LD50 for combination drug was 0.05 pg/ml, TNF-alpha - 9.5 pg/ml, dsRNA>20 jg/ml). Conclusion. The drug containing TNF-alpha and dsRNA molecules may be a promising drug for the treatment of malignant tumors, including melanoma. Key words: tumor n