Индукция гиногенеза в культуре неопыленных семяпочек тыквы

Автор: Шмыкова Н.А., Шумилина Д.в, Кушнерва В.П., Химич Г.А.

Рубрика: Современные направления селекции овощных культур

Статья в выпуске: 1 (10), 2011 года.

Бесплатный доступ

В статье показана перспектива использования культуры неопыленных семяпочек in vitro для получения удвоенных гаплоидов тыквы. Семяпочки 5 гибридов культивировали на питательной среде СВМ. Для индукции гиногенеза использовали два варианта регуляторов роста: первый 0,2 мг/л тидиазурона в сочетании с 0,0001 мкМ эпибрассинолида, второй - 2 мг/л 2,4Д. Частота образования эмбриоидов зависела от варианта сред и сортообразца. Максимальное число эмбриоидов, образующееся из неопыленных семяпочек одной завязи составляло 6-9 штук.

Культура неопыленных семяпочек, гаплоиды тыквы, гиногенез

Короткий адрес: https://sciup.org/14024865

IDR: 14024865

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