Integration and expression of marker genes in chicken embryos with the use of retroviral vector

The efficiency was investigated of transfer of exogenous DNA to chicken embryos in vivo with the use of retroviral vectors. The gene construction involves a marker gene GFT under control of promoter of yearly genes of human cytomegalovirus CMV IE (pLNCgfp) or promoter of Moloney leukemia virus Mo-MuLV (pLgfpSN). Two packing lines GP + envAM12 and PT67 were used for the delivery of retroviral vectors. It was established the high efficiency of embryo transformation with the use of pLNCgfp gene construction (up to 18.8 %). The expression of marker gene was demonstrated in cells of 5- and 15-day age chicken embryos.