Maral artificial insemination applying various sexual heat synchronization protocols

The purpose of research is to test the applied schemes of synchronization of sexual heat in animal husbandry on maral does. Research objectives: to select the optimal stimulating drugs and schemes for synchronizing the sexual cycle of maral does during the rutting period. The study was carried out at the Navotalitskoye State Budgetary Scientific Institution of the Charysh District, Altai Region in 2018–2020. The object of the study were maral does aged 5–9 years, placed at the time of the experiments in a separate park, without the access of stag maral. The sexual cycle of maral does was synchronized with releasing hormones and prostaglandin (Ovsynch program), CIDR – Synch protocol and synchronization with progesterone, prostaglandin and PMSG. The protocols were tested during the rut (September), before insemination, maral does were divided into seven groups of 10 animals. For artificial insemination of maral does, the manocervical method and cryopreserved sperm obtained from stag maral with sperm activity during thawing of at least 3 points were used. Clinical changes were recorded in the form of cervical opening in maral does at the time of artificial insemination. Animals with an open cervix were observed from 20 to 70 % of cases. The result of artificial insemination of maral does was determined by ultrasound scanning. In maral does synchronized according to the Ovsynch and SIDR protocols, pregnancy was observed in 40 % of cases. The control group of maral does and those synchronized according to the protocols PGPG + PMSG were 100% barren. Clinical signs in the form of cervical opening in maral does do not give unambiguous results on the performed artificial insemination due to a number of factors: stress, sperm quality, etc. The approved protocols for the synchronization of sexual hunting, used in animal husbandry, were effective in up to 80 % of cases based on the observed clinical signs of cervical opening in maral does, which makes it possible to use them in maral does breeding with adjusting the timing of artificial insemination.