The use of a nutrient medium 199 for transport of contaminated causative agent of pasteurellosis biomaterial

Taking into account the urgency of the problem of maintaining the viability of microorganisms during the selection of biomaterials before sowing, which is strictly limited and should not exceed 2 hours from the moment of sampling, and in the absence of sampling, the storage and transportation time should not exceed 24 hours. We carried out the present researches which purpose was to reveal possibility of application of a nutrient medium 199 for transportation of the causative agent of pasteurellosis. To achieve this goal was carried out the sampling of biological material from 23 healthy and 78 survivors pasteurellosis calves in 5 affected with this disease farms Pestrechinsky district of Tatarstan. Taking biomaterial was carried out by applying with tweezers discs of filter paper to the mucous membranes of the nasal passages and the oral cavity, followed by immersion in pre-prepared test tubes with a medium 199. The sampled samples were stored in the conditions of farms at temperature 12-18 °C in a darkened room for 2-3 days and sent for research to identify the pathogen...