Usage of polymer nanofibers for investigation of immortalized cells structural anisotropy

HL-1 cels are immortalized adult atrial myocytes which beat spotaneously and expess the main cardiac-specific proteins. The current cells transfected with photosensible ion channels, channelrodopsin, are stimulated with 480 nm laser. In this work, HL-1 cells are cultured on a nanofibrous substrate, which allows us to control the morphology of myocytes and anisotropy of excitation wave propagation. Using the optical mapping technique, we study the behaviour, characteristics and interactions of spiral waves in created anisotropic media.