Studying potato hybrids VSC RAS for the presence of DNA markers of resistance to viruses and cysto-forming nematode

The aim of the study is to identify potato genotypes with genes that control resistance to the potato cyst nematode (Globoder arostochiensis and Globoder apallida), potato virus X (XVC), and Y (YVC) in potato hybrids of the Vladikavkaz Scientific Center of the Russian Academy of Sciences using molecular markers. The analyzes were carried out in the Laboratory of Molecular Genetic Research of the Vladikavkaz Scientific Center of the Russian Academy of Sciences using an instrumentation line for PCR analysis. The breeding material was preliminary evaluated in laboratory and field conditions for the most important economically valuable traits in accordance with the "Methodological recommendations on the technology of the breeding process for potatoes". DNA was isolated from the leaves of potato samples using the DNA-Extran-3 reagent kit for DNA extraction from plant tissues of OOO Syntol (limited liability company). For molecular screening of potato samples, DNA markers of W virus resistance genes were used: STS marker YES3-3A linked to the Rysto gene, and SCAR marker RYSC3 of the Ruadg gene, STS marker Ry186 of the Rychc gene; against the golden potato nematode - SCAR marker of the H1 gene - TG 689, 57 R, N 195, STS marker Gro1-4-1 of the Gro1-4 gene; against the pale potato nematode, the STS marker Gpa2-2 of the Gpa2 gene; and against potato virus X, the STS marker PVX of the Rx1 gene. The combination of Gpa2-2 and PVX markers was found in 9 hybrid combinations. Molecular genetic analysis of potato hybrids showed that the PVX marker of the X virus resistance gene (Rx) was diagnosed in 18 samples. The RYSC3 marker of the Ryadg gene was found in potato hybrids 7/6-18, 7/7-18, 7/16-18.7/21-18, 8/1-18. The H1 gene was confirmed by a combination of three markers TG-689, 57R, N195 in 11 hybrid potato accessions. All studied genotypes lack a specific 602 bp fragment - a marker of the Gro 1-4 gene.