Studying the chemical composition of some genus Tanacetum L. representatives

The purpose of the study is to study the biochemical parameters of three species of the genus Tanacetum: T. balsamita L. (balsamite), T. balsamitoides Sch.Bip. (P. balsamonosa) and T. vulgare L. (P. vulgare). For the study, we used the raw materials of tansy produced by OJSC Krasnogorskleksredstva, a wild-growing type of tansy harvested in the Ufa District of Bashkiria, and we also used the raw materials of balsam tansy and balsam-like tansy, grown in the botanical garden-institute (Ufa). For chemical analysis, plants were harvested at the beginning of flowering, cutting off shoots at a level of 10-15 cm from the ground. The raw materials were dried using the air-shade method under canopies in ventilated rooms, laid out in a thin layer and stirred periodically. The raw materials were packaged and stored in accordance with the requirements of regulatory documentation (OFS.1.1.0019.15) at room temperature in a dry room, not infested with pests, with good ventilation, without direct sunlight. Commodity analysis of raw materials was carried out by generally accepted pharmacopoeial methods according to the following indicators: moisture, total ash and ash insoluble in hydrochloric acid. Qualitative detection of biological active substances was carried out using qualitative reactions and chromatographic research methods. Quantitative determination of ascorbic acid (FS.2.5.01.06.18), tannins (OFS 1.5.3.0008.18) and organic acids (FS.38) in tansy flowers was carried out by titrimetric methods, determination of essential oil content by steam distillation (OFS.1.5.3.0010.15). The commodity indicators of the raw materials of the studied tansy samples: moisture, total ash and ash insoluble in a 10 % hydrochloric acid solution do not exceed the content standards specified in the pharmacopoeial monograph. The chemical composition of the studied tansy species contains valuable biologically active substances, among them essential oils, tannins, ascorbic acid, and organic acids, which may allow the use in scientific medicine not only of T. vulgare, but also of T species used so far only in folk medicine balsamita and T. balsamitoides, which successfully passed the introduction test at the South Ural Botanical Garden-Institute of the Federal Research Center of the Russian Academy of Sciences.