The study of red blood cells by scanning electron microscopy

Introduction. Using the possibilities of modern equipment, such as a scanning electron microscope (SEM), could provide important additional information for red blood cells (RBCs) diagnostics, which can be used to introduce new methods of complex studies of blood tests in the diagnosis of renal disease with hematuria syndrome. Materials and Methods. Investigations of the morphology the RBCs of patients with hematuria by SEM high-resolution instrument JSM-7800F. The technique of cell and uncoated nanoparticles research without conductive coatings has been developed. Results. The findings obtained results of a comparative analysis of the methods of mathematical statistics, linear dimensions of the red blood cells of children Berger's disease patients and control group. Nanoscale particles detected on the surface of red blood cells and IgA-nephropathy. Smear of blood samples showed that in certain diseases of the observed changes in the morphology of red blood cells and the presence on their surface nano-objects. It can be assumed that these objects are of organic origin, as many organic objects are dielectrics and in the study of SEM, they can be seen as bright glowing objects. Nano-objects have dimensions similar to the small size of viruses and can be a confirmation of the assumption of a possible viral etiological factor Berger disease and other forms of nephropathy. The changes in linear dimensions of erythrocytes upward Berger in diseases compared with the size of the control group and the group of erythro-cytes of patients with other forms of nephropathy. Discussion and Conclusions. The SEM method without spraying of the samples is very accurate for the study of the morphology of blood cells. Its use will improve the quality of diagnosis is difficult diagnosed Berger disease. A further increase in statistics and the analysis of nanometer-sized objects extend the knowledge of the manifestations of the disease.