Study of methods of preparation of water extracts from propolis and propolis water

The purpose of the study was to investigate the technology of preparation and biological activity of aqueous extracts of propolis and propolis water from propolis residues after alcohol extraction. The studies were carried out in the laboratory of the Federal State Budgetary Scientific Institution "Federal Research Beekeeping Centre". The object of the study was: native propolis, propolis residues after alcohol extraction, aqueous extracts of propolis, propolis water. Such objects of the study as aqueous extracts of propolis, propolis water were obtained by maceration (with and without heating). The study of the biological activity of aqueous extracts of propolis and propolis water was carried out according to GOST 28886-19, TU 9882-027-00669424-2015. The influence of temperature, extraction time, the degree of grinding of raw materials, mixing on the amount of biologically active compounds in the aqueous extract of propolis were studied. The conditions for the preparation of propolis water from propolis residues after alcohol extraction, the content of biologically active compounds in propolis water were also studied. Propolis water was stored at t = 6 ± 2 °C, the physicochemical parameters of freshly prepared propolis water were analyzed after 10 days and after 1 month of storage. The aqueous extract of propolis was stored at t = 6 ± 2°C, the physicochemical parameters of the freshly prepared propolis extract were analyzed after 1 month of storage, half a year and a year of storage. The biological activity of aqueous extracts of propolis and propolis water during storage was investigated. In the study of various methods of extracting propolis, the database of the content of biologically active substances in propolis water and aqueous extracts of propolis was replenished. Propolis water can be obtained from propolis residues after alcohol extraction, which improves the degree of processing of propolis. An increase in the extraction temperature, an increase in the extraction time increases the yield of biologically active propolis compounds into the solution. The use of shungite water increases the shelf life of the water extract of propolis. Aqueous extracts of propolis, propolis water are promising biologically active products