Research the microbic profile of the structured associative culture of microorganisms – kefiric grains

The microbic profile of kefiric grains from four technogenic niches is investigated. At research 55 isolates of yeast it is not revealed the cultures, possessing β-galactosidase activity, capable to use lactose for spirit fermentation. At research 39 isolates of bacteria presence of two physiological groups of lactic bacteria is revealed: possessing and not possessing β-galactosidase activity. PCR method used for identification of the allocated lactic bacteria showed that using the methods through «allocation of pure cultures» doesn't allow to receive the objective characteristic of microbic profile of kefiric grains. Comparison of grains from two technogenic niches when using a method of denaturing gradient gel electrophoresis («without allocation of pure cultures») showed their identity on structure of lactic bacteria and prospects of using the method for development the conceptual model of biological system of kefiric grains.