Studying the osteoplastic properties of bone tissue biomineral

Aim of study – morphological analysis of the osteoplastic effectiveness of bone tissue biomineral (BTB) obtained without thermal and demineralizing processing, as well as studying the mechanisms of reparative osteogenesis for BTB implantation into bone defects. An experimental-and-morphological study was performed on 30 mature Wistar rats of control and experimental groups, in which perforation defects were produced in the proximal third of tibial shaft. In the experimental group an implant was inserted into the site of defect – granulated BTB. Surgical interventions were performed under general anesthesia. Animals were withdrawn from the experiment after 1, 2, and 3 weeks. The bones operated were fixed and embedded in paraffin and araldit. Histological sections were stained with hematoxylin-eosin and with picrofuchsin by Van Gieson. The chemical composition and surface microrelief of BTB granules were investigated using an electron-probe microanalyzer and a scanning electron microscope, the intensity of osteogenesis process was determined, as well as the degree of maturity of newly formed bone tissue in regenerated bone. The results of the study performed evidence the fact that early intense angiogenesis is provided, activation of reparative posttraumatic osteogenesis occurs, acceleration of both remodeling and organotypical restoration of the bone involved take place in case of BTB implantation into bone defects.