Instrumental error sources in quantitative absorption spectroscopy

The paper discusses how to identify and assess the impact of instrumental errors caused by non-uniformity of the energy spectral efficiency of the monochromator diffraction grating and the non-linearity of the spectral sensitivity of the spectrophotometer detector, for the absorption spectra and the optical density of the samples study. It is shown that, due to these causes may be distorted the absorption band contour and absorption band area, even when spectrophotometer operates in the standard mode and recommended range of the optical sample density scale.