Study of the influence of intravenous injection in rats Na 2EDTA and polyglucinum on breathing restore and blood pressure at deep hypothermia

The effect of intravenous injection Na 2EDTA and poliglucinum (dextran) on breathing, and blood pressure levels in the terminal stage of deep hypothermia after the arrest of breathing has been studied. White rat males of Wistar line were placed in the water of 9-10°C. Respiratory arrest took place at a temperature in the rectum of 15-16º C and in the brain of 16.5-18ºC. Rats then were removed from the water; rat from the first group were injected with Na 2EDTA, those from the second - with poliglucinum, and animals from the thirds - with both drugs. Control animals, who did not receive any injections after the extraction from the water, have died. On the contrary, all injected rats started breathing. Respiration frequency in rats injected with Na 2EDTA rose up to 25-30 cycles/min; that in rats, injected with poliglucinum - up 5-12 cycles/min. Injection of poliglucinum helped to maintain the blood pressure at the level of 60-80 mm Hg at low body temperature (13-14ºС). It is shown that joint application of Na 2EDTA and poliglucinum provides more rapid restoration of breathing after its arrest. However, the main factor, which stimulates breathing after its termination due to deep hypothermia is the reduction of calcium ions concentration in the blood.