Research of capabilities of median filtering for digital image processing of populations of local biological objects

The tasks solved by median filtering in measuring sizes of the local biological objects that are small comparing with the full images frame size are listed. The object image distortions caused by median filter are determined. A method for correcting the measured size of the object after median filtering of the image is proposed. An example of experimental determination of size of the granulocyte (blood cells) from its microscopic image is given, which shows that the median filtering is a convenient and reliable tool for digital image processing in the measurement of parameters of local objects.