Differences in spectra of fibrinopeptide derivatives in blood plasma after exposure to o-isobutyl-s-2-diethylaminoethyl methylphosphonothiolate

It is a well-known fact that organophosphates (OP) inhibit several major enzymes, and this fact can be easily proved by simple laboratory and clinical biochemistry methods. On the other hand, diagnostics of low dosed chronic OP action, or their delayed effects after subacute intoxication is a serious problem because of lack of inhibition or recovery of the enzymes activity. Determination of peptide or serum blood plasma spectrum can serve an alternative and more sensitive method for intoxication diagnostics. In this paper, we demonstrate data on peptide spectra of rat plasma or serum after subchronic intoxication with Russian VX (RVX) in vivo, and that of human plasma or serum after human blood exposure to RVX in vitro. It is shown that the principal peptide plasma or serum component after RVX exposure is peptides derivatives of fibrinopeptide A, thus showing inactivation of exopeptidases under RVX exposure.