ИЗОТЕРМИЧЕСКАЯ ПЕТЛЕВАЯ АМПЛИФИКАЦИЯ LAMP В ФОРМАТЕ МИКРОУСТРОЙСТВ (ОБЗОР)

А. Н. Зубик; Г. Е. Рудницкая; А. А. Евстрапов; А. N. Zubik; G. E. Rudnitskaya; A. A. Evstrapov

doi:10.18358/np-31-1-i343

Научные статьи \ Математика. Естественные науки \ Биологические науки в целом \ Материальные основы жизни. Биохимия. Молекулярная биология. Биофизика

ИЗОТЕРМИЧЕСКАЯ ПЕТЛЕВАЯ АМПЛИФИКАЦИЯ LAMP В ФОРМАТЕ МИКРОУСТРОЙСТВ (ОБЗОР)

Автор: А. Н. Зубик, Г. Е. Рудницкая, А. А. Евстрапов

Журнал: Научное приборостроение @nauchnoe-priborostroenie

Рубрика: Приборостроение физико-химической биологии

Статья в выпуске: 1 т.31, 2021 года.

Бесплатный доступ

Изотермическая петлевая амплификация (LAMP) широко применяется в качестве быстрого и чувствительного метода диагностики и наиболее перспективна для проведения анализа в условиях ограниченных времени и ресурсов, например в местах оказания медицинской помощи. В работе рассмотрены основные принципы работы LAMP, способы детектирования продуктов реакции, а также микрофлюидные устройства и аналитические системы на основе LAMP, в том числе разработанные для экспресс-диагностики в условиях пандемии COVID-19.

Микрофлюидные устройства, изотермическая петлевая амплификация, LAMP, COVID-19, point-of-care (POC)

Короткий адрес: https://sciup.org/142226565

IDR: 142226565 | УДК: 577.2.08 | DOI: 10.18358/np-31-1-i343

LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) TECHNIQUE IN MICRODEVICE FORMAT (REVIEW)

Loop-mediated isothermal amplification (LAMP) is widely used as a fast and sensitive diagnostic method and is most promising for analysis in conditions of limited time and resources, for example, in places of care. The paper discusses the basic principles of LAMP technique, methods for detecting reaction products, as well as some microfluidic devices and analytical systems based on LAMP, including those developed for express diagnostics in the context of the COVID-19 pandemic.

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