The study of allele composition of gliadin-coding loci of durum wheat selection lines

The aim of the research was to study selection lines of spring durum wheat to determine their genetic structure and origin. The material for the study was the grains of Bezenchukskaya stepnaya variety (hordeiforme form) and 4 selection lines (leucurum) isolated from its crops. Since the cause of the appearance of plants of another variety could be mechanical clogging, the variety Marina was studied as a likely source of varietal impurity. It was found that none of the studied lines on the component composition of gliadin corresponded to Bezenchukskaya stepnaya variety. In selection lines № 1 and 2, the type of spectrum prevailed, which was identical to the II biotype of the variety Marina (95.45 and 93.18 %, respectively). According to the component composition of gliadin, selection lines № 3 and 4 were close to the spectra of the variety Marina. Genetic formula of gliadin line№ 3 Gli-A1d g; B1d la; A2d a; B2d a, the main biotype of line № 4 - Gli-A1d g; B1d la; A2d d; B2d a. The genetic distance according to Dice between the II and IV biotypes of the variety Marina and lines № 3 and 4 was 0.2. The genotypes of these lines were natural hybrids or mutant forms, in the formation of which the variety Marina was involved. The genotypes from which the studied selection lines were obtained appeared in the crops of Bezenchukskaya stepnaya variety as a result of mechanical clogging. However, taking into account that lines № 3 and 4 had been characterized by high grain quality and unique gliadin spectra; they were left for further study and inclusion in selection process.