Researching of the anti-free radical activity of propolis, rhodiola rosea and horseradish

The purpose of the work was to study the anti-free radical activity of agents obtained from propolis, Rhodiola rosea and horseradish in a model system of lipid peroxidation (LPO). The effectiveness of the antioxidant action of propolis, Rhodiola rosea and horseradish was determined in model lipid peroxidation systems in liposomes. Liposomes were prepared according to the following scheme: in order to obtain a lecithin solution (10%), 0.5 g of soy lecithin was added to 5 ml of 96% alcohol, then the resulting mixture was heated to 30-40°C, while vigorously stirring until a homogeneous consistency was obtained. . The production of liposomes was carried out by the injection method, in which 0.25 ml of a 10% lecithin solution was quickly injected into 5 ml of distilled water using a syringe with a thin needle, constantly and intensively stirring. Based on the results of the study of MDA in model systems, it was found that in the system with the addition of propolis extract and propolis with Rhodiola rosea, MDA values were lower than in the control system by 25.00% and 27.00%. And when studying extracts from boluses No. 1 and No. 2, malondialdehyde decreased compared to the control system by 30.00% and 33.00%, respectively. When studying the effect of drying on the antioxidant properties of horseradish, it was found that preparations based on horseradish leaves without drying (No. 1 and No. 2) have the greatest anti-free radical effect, although preparations based on dried leaves show sufficient anti-free radical activity. Thus, a decrease in anti-free radical activity in preparations based on dried leaves without infusion was established by 7.7% (decoction) and 10% (infusion); on the first day of infusion by 5.4% (decoction) and 10.4% (infusion); on the second day of infusion by 2.9% (decoction) and 7.1% (infusion); on the third day of infusion at 5% (decoction) and 12.5% (infusion). The results of studies conducted to study the antioxidant properties of products based on propolis, Rhodiola rosea and horseradish in a model system of lipid peroxidation confirmed the presence of the desired properties, which makes it possible to recommend these plants to livestock production for use as a source of antioxidant agents.