Study of the effect of b-cyclodextrin complexes with levofloxacin on some microbiological parameters of wounds and large intestine in sturgeons

The aim of research is to study the effect of complexes of silica gel-chitosan-β-cyclodextrin and silica gel-β-cyclodextrin with levofloxacin on some microbiological parameters of incised wounds and the large intestine of sturgeons. The experimental study was carried out on the basis of the research laboratory Progressive biotechnologies in aquaculture of the Saratov State University of Genetics, Biotechnology and Engineering named after N.I. Vavilov. The therapeutic effect of the silica gel-chitosan-β-cyclodextrin complex with levofloxacin in relation to incised wounds was determined on yearlings (hybrids of Russian and Siberian) sturgeon when this complex was added to the feed at a concentration of 16 % levofloxacin (0.99 mg per 1 kg of fish weight) for 5 days of treatment. The prophylactic properties of the silica gel-β-cyclodextrin complex with levofloxacin were studied at 5 % levofloxacin (0.35 mg per 1 kg of fish weight) for 10 days of prophylaxis. For this, 4 groups of sturgeons (n=10) were formed: control, 1st experimental - fish with a wound and treatment (silica gel-chitosan-β-cyclodextrin with levofloxacin was used for treatment); experimental 2 - fish that received a prophylactic silica gel-β-cyclodextrin complex with levofloxacin in order to prevent possible skin diseases and disorders in the gastrointestinal tract; 3rd experimental - fish with a wound without treatment. The wounds were modeled by dorsal incisions (2 cm long) of the skin. Washings from the surface of the wounds were taken with sterile cotton swabs. The contents of the large intestine were removed under sterile conditions after opening the fish. The total microbial count (TMC) and the number of lactic acid bacteria were determined by the method of serial dilutions, by counting the colonies after cultivating the crops for 72 hours at 30 °C on meat-peptone agar (MPA) and at 37 °C on MRS-agar, respectively. Studies have shown that complexes of silica gel-chitosan-β-cyclodextrin, silica gel-β-cyclodextrin with levofloxacin have a positive effect on the treatment of incised wounds in sturgeon and their intestinal microflora, contributing to the normalization of TMF, bringing this indicator closer to the values of TMF of the control group of fish, and does not lead to significant changes in the number of lactic acid bacteria in the intestines of sturgeons.