The study of genetic diversity of wild pomegranate (Punica granatum L.) genotypes of Azerbaijan using ISSR markers

The research objective was studying the genotypes of wild pomegranate of Azerbaijan from 6 geographical areas. The research was conducted in the Laboratory of Biotechnology of Institute of Genetic Resources of National Academy of Sciences of Azerbaijan in 2015-2018. The objects for the research were 90 samples of pomegranate which fragments of leaves collected from 6 regions of Azerbaijan. In total 110 PCR fragments were identified, of which 86 were polymorphic.The number of amplified fragments per locus varied from 5 to 10, and the fragments obtained were in the size range of 100-1100 bp.On average, one primer initiated the synthesis of 7.8 fragments. The quantity of polymorphic fragments of DNA varied from 3 to 9. The minimum number of polymorphic fragments was identified by primers of UBC 827 and UBC 857 which average number made 6.1. Depending on the primer the quantity of polymorphic loci fluctuated within 57-100 %, the level of polymorphism averaged 78 %. The index of genetic variety (IGV) on each ISSR to the locus which average value made 0...