Study of genes controlling dehalogenation in bacteria-destructors of 4-chlorbenzoate

Screening of bacteria-destructors of 4-chlorobenzoic acid for fcb-genes controlling the initial stage of 4CBA destruction (dehalogenation) has been made by polymerase chain reaction. It was revealed that strains Arthrobacter sp. H4, H5, Pseudomonas fluorescens H2, Micrococcus sp. G120, and Rhodococcus ruber P25 comprised fcbA and fcbB genes encoding 4CBA-CoA-ligase and 4CBA-CoA-dehalogenase, respectively. The analysis of restriction fragment length polymorphism and comparing of sequenced regions of fcb-genes of Rhodococcus ruber P25 with similar sequences showed 95-99% affinity to fcb-genes of strains-destructors of 4CBA from genus Arthrobacter