Studying the effect of aquafoods with haprin on morphobiochemical parameters of sterlet blood (Acipenser Ruthenus)

To study the effect of aquafoods with haprin on the morphobiochemical parameters of sterlet blood, a series of experiments were conducted and the biological fluid was taken in the conditions of the center "Breeding of valuable Sturgeon breeds” of the Volgograd State Agrarian University. Blood sampling was carried out from the caudal vein, by crossing it. Hematological analysis included determination of hemoglobin concentration, total number of erythrocytes, leukocytes, hematocrit, total protein in blood serum, glucose. Biochemical studies were carried out according to the following indicators: ALT, AST, albumin, bilirubin, creatinine, urea, total calcium, iron. The experimental material obtained was biometrically processed using Microsoft Office software. As part of this process, an application was used to determine the criterion for the reliability of the difference in the Fisher Student. The inclusion of aquaculture feeds in the sterlet's diet together with the high-protein drug haprin did not have a pathological effect on the condition of the fish. Haprin had a positive effect on the processes of erythropoiesis – the manifestation of an increase in hemoglobin and an increase in the number of red blood cells. In the control group where haprin was not used, the hemoglobin level and the number of red blood cells were 85.9 g/l and 0.51x 1012/l, in the 1st experimental group – 91.0 g/l and 0.55 x 1012/l; in the 2nd experimental group – 91.2 g/l and 0.55 x 1012/l; in the 3rd experimental group – 91.8 g /l and 0.56 x1012/l. The inclusion of food in the diet of the experimental fish groups together with the drug haprin led to an intensification of protein metabolism compared with the control: in group 1 – by 16.6%, in group 2 – by 17.6%, in group 3 – by 21.4%. The composition of the blood obtained from the fish of the experimental groups differed from the control in terms of enzyme activity: in the experimental samples, the activity of ALT and AST was higher than in the control group: in groups 1, 2 and 3 – 7.01, 20.56 and 23.36%, as well as 2.32, 0.84 and 3.37%, respectively. The concentration of calcium and iron in the blood of experimental fish was at the same level (2.7–2.9 mmol/l; 29.9–30.3 mmol/l, respectively)