The effect of hydroxylapatite nano-scale particles on bone marrow hemopoietic precursors in vitro

The potential of nano-scale particles of hydroxylapatite (HAP), its derivative with copper and zinc inclusion into apatite structure (HAPCu,Zn), synthesized by the mechanochemical method, to influence the colony-forming activity of hemopoietic progenitor cell pool has been studied in vitro. HAPcu.zn has been established to stimulate the output and proliferative activity of precursors, identified by routine staining as granulocytic colony-forming units (GCFU-G) in bone marrow liquid 7-day culture of CBA/CaLac mice. Besides, (HAPCu,Zn) increased the cloning effectiveness of monocyte progenitor cells (CFU-M) as well. But, at the same time, synthetic stoichiometric HAP had no such an effect. The effect absence was not connected with cytotoxicity of the samples studied. Stoichiometric HAP didn't demonstrate bioactive properties in case of five-week dissolution in model biological liquid (isotonic solution of sodium chloride). At the same time (HAPCu,Zn) alkalized a solvent due to cations' coming out from molecular matrix. Thus, one of the mechanisms of colony-forming potential increase of bone marrow progenitor hemopoietic cells is connected with the effect of (HAPCu,Zn) nano-particle dissolution products