Efficiency of androgenetic haploid production depending on a mode of plant regeneration, medium composition and anther inoculation density

The possibility of the one step plant production in spring barley anther culture in vitro via agar substitution for native corn starch obtained from the seeds of line which was a carrier of the mutant endosperm structure gene su 2 and a comically modified starch D-5a has been investigated. The effect of anther inoculation density on the efficiency of the haploid production process has also been studied. It has been shown that on the media possessing starches the lowering of the regeneration frequency was conditioned by a high intensity of nonembryogenic callus growth (su 2 - starch) or by a suppression of embryoid growth and development (chemically modified starch). A reduction of anther inoculation density has promoted a frequency of plant regeneration on agar solidified medium.