Experimental study of peculiarities of formation of vibrio parahaemolyticus biofilms on biotic surfaces of objects

The most common pathogen found on seafood and capable of causing serious diseases in humans are, V. parahaemolyticus. Occupying different ecological niches, V. parahaemolyticus due to the presence of one polar flagellum can exist in water in plankton form or be attached to inert particles such as zooplankton, fish, mollusks and crustaceans and form biofilm. The aim of the work was to study the features of the formation of biofilm by V. parahaemolyticus on the surfaces of biotic objects for further improvement of the algorithm of research on halophilic vibrions in recreation areas at the stage of sampling. The study of the features of the formation of the biofilm by vibrions was carried out by bacteriological method and Real-Time PCR. It is shown that the utilization of V. parahaemolyticus actively use as nutrients the components of scales and chitin, which contributes to the formation of biofilms and intensive reproduction on biotic surfaces. The high concentration of viable cells of V. parahaemolyticus in the biofilm on the surface of biotic substrates should be taken into account when choosing the objects of study for the presence of V. parahaemolyticus, especially in recreation areas.