Experimental modeling of bone defect with a sclerosal wall

Purpose. To develop a model of bone defect with a sclerosal bone wall, suitable for studying various pathogeneses of the diseases related to bone sclerosing, as well as for studying their consequences. Materials and Methods. 12 rabbits divided into two (2) groups were used in the experiment. The experimental group included the animals underwent modeling bone defect with a sclerosal wall (n=6). Bone defect without a sclerosis zone produced in the control group of animals (n=6). Radiological and morphological studies performed in the both groups by Day 45 and Day 90 after the surgeries made. Euthanasia in order to take organs for morphological investigation performed on Day 45, Day 90 after surgery, for three animals in each period, respectively. Results. The percentage of fibrous tissue in the control group with unchanged bone defect was 3.33±0.8 %, and in the model with a sclerosal defect wall the ratio of fibrous tissue was substantially higher - 26.7±5.2 %, resulting in sharp retardation of regeneration rate comparing with the control group. Conclusion. We have developed an experimental model of bone defect with a sclerosal wall confirmed radiologically and morphologically, which allows to study the processes of biodegradable bone-substituting material reorganization. The combined influence as a thermal burn and cement packing contributes to forming the zone of sclerosis and fibrosis around the bone cavity formed.