ELECTROCHEMICAL DETECTION OF SINGLE DNA MOLECULES WITH A SOLID-STATE NANOPORE

We demonstrated the electrochemical detection of single DNA molecules using a thin SiN membrane with a nanopore. Experiments were aimed at a record of an ion current for a buffer solution without/with 10k, 5k, and 500 b.p. DNA molecules. Obtained data was analyzed by a developed algorithm for the detection of translocation events through the nanopore. This algorithm allows us to analyze the amplitude, duration, and frequency of detected events in order to analyze the properties of the detected molecules. In the case of 5k and 500 b.p. DNA, a nonlinear dependence of the translocation time on their length is observed. It is shown that a 10-fold decrease in the length of molecules leads to a 35% decrease in the translocation time. The translocation detection possibility sets the basis for the future development of a solid-state nanopore sensor.