Mapping of an epitope of Erbb-2 for recognizing by monoclonal antibody 4b8 using phage display technology

This article presents identification and mapping of an epitope in the protein Erbb-2 which binds to the monoclonal antibody 4b8 intended for use in immunochemistry to estimate the level of Erbb-2 production. Epitope was identified by biopanning of phage display library based on filamentous phage. Location of the epitope was revealed in ClustalW program, analysis of specificity was carried out by ELISA. The epitope is also similar to the sequence on tyrosine kinase domain. It suggests a possible interaction of this site with 4b8.