Clinical value of indicators of volatile fatty acids of blood for diagnostics of pancreatic necrosis

Volatile fatty acids (VFA) are metabolites of facultative anaerobic and obligate anaerobic bacteria. The importance of anaerobic microflora and its metabolites for the diagnosis of pancreatic necrosis (PN) has not been sufficiently studied to date. The purpose of the study was to evaluate the informative value of the volatile fatty acid indicators for the diagnosis of PN. Materials and methods: The study included 35 patients with PN. After establishing the diagnosis of PN, 35 blood samples of patients with PN chroma- tography were studied. Analysis of the concentrations of VFA: acetic, propionic, oily and isovaleric by gas-liquid chromatography on an automated gas chromatograph “Crystallux-4000” with a capillary column “HP-FFAP” and a flame ionization detector. Results: Concentrations of acetic, propionic, butyric acids and the amount of VFA are higher, and the anaerobic index and the concentration of isovaleric acid in patients with PN are lower in comparison with those of practically healthy donors...