Integrated assessment of the influence of nutrient environment and the temperature on the conservation of garden strawberry in vitro

The research objective was studying the influence of nutrient medium and temperature condition on the preservation of garden strawberry in vitro. On the basis of the analysis of the obtained data the factors (the temperature and structure of nutrient medium) were defined and their complex assessment for the development of parameters of medium-term storage of valuable genotype of garden strawberry variety Nashe Podmoskovye in vitro culture was given. The distinctions in the effect of hexatomic alcohols (mannitol and dulcite) depending on the temperature on the viability of regenerated plants of garden strawberry were established. The addition of osmotically active substances in the concentration of 0.45 % in the medium contributed to the preservation of viable explants of strawberries at the temperature of + 22...24 °C for 12 months on the medium with mannitol (56.5 %) and 9 months on the medium with dulcite (25.0 %). Dulcite did not have a significant positive effect on the preservation of microtubes under standard cultivation conditions. The proportion of viable explants of strawberries was below the control option throughout the conservation period. It was shown that under low positive temperature (+ 4...6 °C) conditions the increase in the period of direct cultivation was observed from 3 months for the explants on the medium with dulcite to 6 months on the medium with mannitol. The total share of viable explants at the temperature of + 4...6 °C was 10-40 % after 12 months of deposition. In control variant the viability of microprobe ranged from 70.0-73.3 %. Optimal source of carbohydrate nutrition for the variety Nashe Podmoskovye micrun shoots at the temperature of + 22...24 °C was mannitol at the concentration of 0.45 %; at +4...6 °C - sucrose at the concentration of 3.0 %. The conservation of explants of the variety Nashe Podmoskovye was significantly affected both by the nutrient medium and cultivation conditions.